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For the Cytochrome-C release assay, cells were seeded on coverslips in a 24-well plate and incubated for 5d total with the pan-caspase-inhibitor QVD-OPH (20 M in 500l)

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A research team in the Netherlands is further evaluating its long-term effects and safety ( 6.4 Dietary interventions Diet and nutritional supplements play a crucial role in shaping the diversity and function of the gut microbiota