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Kilobase ladder
Storage Buffer: 50% glycerol containing 25 mM sodium acetate (pH 4
then induce to high copy number for DNA recovery
Unit Definition: One Molecular Biology Unit (MBU) of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutes at 37°C under standard assay conditions
0 × 108 145-kb BAC Clone Not tested 7 × 107 13
E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects Kilobase ladderTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES
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