Expressional level of these genes were decreased in EPA and EPA + L-carnitine group (Fig
10.1074/jbc.M803287200 66 Lapuente-BrunE.Moreno-LoshuertosR.Acin-PerezR.Latorre-PellicerA.ColasC.BalsaE.et al
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Relative band pixel intensities were semiquantitatively analyzed using ImageJ software (version 1.8.0, National Institutes of Health, Bethesda, MD, United States), and protein loading was normalized using an antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-32233, Santa Cruz Biotechnology, Santa Cruz, CA)
The PXR-induced sensitivity was associated with decreased activities of superoxide dismutase and catalase, enzymes that scavenge superoxide and hydrogen peroxide, respectively [25]