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During the process of making ATP, your mitochondria naturally produce free radicals as a byproduct

Concurrently, decreased levels of antioxidant antimicrobial metabolites in PCOS further compromise cellular reducing capacity and intensify redox imbalance

They are better understood as complementary research tools than as alternatives

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in order to limit time to freezing (and potential changes in metabolic phenotype due to death) no additional processing of tissue occurred and organs were not perfused prior to collection