Food Chemistry: X 21, 101103
Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h
Allergic Reactions Signs of a possible allergic reaction include: Rash Hives Severe itching Swelling of the lips, tongue, or throat Difficulty breathing These symptoms require immediate medical attention
Seek medical treatment to the infected areas in your body
Traveling with reconstituted peptides For researchers who travel or need to transport reconstituted peptides, maintaining the cold chain is critical