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Real-time quantitative PCR (RT-qPCR) for evaluation of defense responses RNA was extracted from all samples using the GeneJET Plant RNA Purification Mini Kit (Thermo Scientific, USA), following the manufacturer's instructions

Via The Big Lead is the league's policy on tobacco and alcohol on game day: Players and other club and League employees (including game officials) must not use alcohol or tobacco products while in the playing field area or while being interviewed on television

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Schedule I has the highest abuse risk and no accepted medical use