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However, methylated nucleosides, which are abundant in tsRNA, often result in pauses, stops, or misincorporations during reverse transcription 6,7,8

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The detailed protocol and details of cellular differentiation (Club cells, goblet cells, basal cells, ciliated cells, etc.) of the bro-ALI model has been described previously and the model has been in several studies 34, 35, 37,38,39,40,41