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Paq5000 DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) was used for amplification from swab samples, and 5% dimethyl sulfoxide (DMSO) was added to the reaction to improve the concentration of the amplified products from the metagenomic template

Supplementary material The Supplementary material for this article can be found online at: Footnotes 1.^In November 2018, FDAs commissioner made an initial statement proposing new steps to reduce youth access to ENDS, including reducing access to flavored ENDS (other than mint, menthol, tobacco) (U.S

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Engineered for addiction Its no accident that modern cigarettes are so powerfully addictive