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For differentiation of mouse SVF cells, the cells were first cultured to confluence in DMEM/F12 medium supplemented with 10% FBS and 1% P/S, induced to differentiation in the adipogenic cocktail consisting 1 M dexamethasone, 5 g/ml Insulin, 0.25 mM IBMX, 5 M rosiglitazone, followed by maintenance treatment with 1 g/ml Insulin and 5 M Rosiglitazone every 48 h until fully differentiated

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(PubMed) Evans JR, Lawrenson JG