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The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 20% methanol in dichloromethane to produce SS(CAM-TPP) 2 (92 mg, 32%) as a beige fluffy/foamy solid

Additionally, it suppresses the antiapoptotic protein Bcl-2 and upregulates the proapoptotic protein Bax, thereby inhibiting abnormal thyroid cell proliferation (101)

Insoluble material was removed by centrifugation, and the cell lysates were combined for 2 h at 4 C with the recombinant GST-fusion proteins bound to glutathione-sepharose beads

Analysis of GSH and GSSG after derivatization with N-ethylmaleimide