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Centrifuge at 8000 g for 10 minutes, take the supernatant and place it at 4 for test

That is a unit conversion, not a recommendation about what to use

Fasting is required for rescuing hippocampal-dependent long-term memory in both sexes We examined the contribution of fasting on CR-induced improvements in cognition by performing behavioral assays on 12-month-old 3xTg and NTg mice that had been fed either an AL, DL, or CR diet prior to sacrificing them for the histological assays described above

this is more of a supportive measure than an interaction concern

The oxidative stress level was increased in GLA-mutant kidney organoids, and the increase was accompanied by apoptosis