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4 Prevalence, classification, and mechanisms of action of anabolicandrogenic substances Although a uniform classification of anabolicandrogenic substances (AAS) has not been formally established, in practice a functional division based on chemical structure, pharmacokinetics, and route of administration is commonly applied, as illustrated in Figure 1

This keeps the work aligned with regulatory guidance while still allowing for detailed exploration of how the peptide behaves in various models

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The RNA was then reverse-transcribed into cDNA using the PrimeScript RT reagent kit (Takara, Dalian, China) ( -CT method

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