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Then 1 g of trypsin was brought up in 20 l of 100 mM TEAB and added to the S-Trap, followed by an additional 20 l of TEAB to ensure the sample did not dry out
1 Introduction Iron is an essential element required for numerous biological processes in the human body, including oxygen transport, energy production, and enzymatic reactions ( Cell death is a fundamental biological process that occurs in both physiological and pathological conditions ( Kidney diseases, including acute kidney injury (AKI) and chronic kidney disease (CKD), are common and significant public health concerns ( 2 Materials and methods 2.1 Study subjects This study used the setting and the subjects from the CKD screening project of the Chronic Kidney Disease in the Northeast Thailand project, as described elsewhere ( 2.2 Measurement of protein in serum and urine The serum was sent to the clinical chemistry laboratory at Srinagarind Hospital, Khon Kaen, Thailand
