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PMID: 38367056 J Cell Mol Med CD44/ERM/F-actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis

The hypothesis is that a local application might more rapidly encourage the growth of new blood vessels in an area that may have poor blood supply, which is a common issue with tendon and ligament injuries

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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

Do not use cloudy, discolored, or particle-containing solution unless the official instructions say otherwise