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It's important to ensure that the rubber stopper and the locking device are aligned properly, with the steel straw extending below the metal cage lid

The authors thank the Cryo-Electron Microscopy Center (SUSTech), cryo-EM (KEMC) and advanced mass spectrometry facility (KMS) of the Kobilka Institute of Innovative Drug Discovery (CUHKSZ) and SUSTech Core Research Facilities for technical assistance

10.3389/fphar.2024.1415971 117 SongD.HaoJ.FanD

Expert Insight / Pro Tip Analytical Pro-Tip: When planning multi-phase laboratory models, always purchase your entire sample set from the exact same manufacturing batch