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Glutathione is synthesized in two sequential enzymatic reactions: glutamate cysteine ligase (GCL) catalyzes the first, rate-limiting step, and glutathione synthetase couples -glutamylcysteine to glycine in the last step [13]

This feature of DFO makes it a selective chelating agent for iron and aluminium, and following chelation, it results in molecules of ferrioxamine and aluminoxamine, respectively being formed

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