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Animal models demonstrate increased fibroblast density and type I collagen expression in BPC-157-treated groups compared to controls. Sikiric, P

Although effective, these protocols were lengthy, costly, and at times impracticalespecially for patients needing intermittent therapy, as serial desensitization was required for temporary tolerance for each antibiotic course

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Immunofluorescence Analysis The cells were fixed for 30 min at room temperature with 4% of paraformaldehyde in 0.1 M PBS, 40 pH 7.4, and permeabilized with 0.1% of Triton1-X100 in PBS for 10 min, followed by blocking with 5% skimmed milk in PBS for 30 min