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Degenerated menisci produce catabolic mediators such as prostaglandin E2 (PGE2), nitric oxide, and cytokines that can diffuse into synovial fluid and exacerbate articular cartilage degeneration (Krupkova et al., 2018)

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Finally, the pictures were captured using a Leica DM4000 B epifluorescence microscope (Leica Microsystems, Wetzlar, Germany), and the relative fluorescence intensity was calculated using Image-Pro Plus 6.0 software (Media Cybernetics, Rockville, MD, USA)

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