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Flies were homogenized for 15 s using a Teflon micropestle and the homogenate centrifuged at 14,000 g for 2 min

To enhance the precision of extractant loss estimations attributable to solubility and gain a deeper understanding of the factors influencing third-phase formation in liquidliquid extraction, we also identify and quantify impurities present in commercially available D2EHPA

First, exploring the long-term effects of L-carnitine supplementation on endurance performance would be beneficial, as this study was limited to short-term supplementation