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Originating from ectoderm-derived neural stem cells in the neural tube, astrocyte lineage commitment begins with radial glia cells, which express classical markers such as GFAP, glutamate aspartate transporter (GLAST) and calcium-binding protein (S100B) 1

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For immunofluorescence, sections were blocked with 5% fetal bovine serum (FBS) and incubated overnight with primary antibodies (1:100 dilution) at 4C

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Microcirculation 10 , 391400