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Samples (20 l) were mixed with 5 l of 5x loading buffer (0.3 M Tris-HCl pH = 6.8, 25% -mercaptoethanol, 50% glycerol, 10% SDS, 1% bromophenol blue), heated to 95 C for 5 min, and 15 l of samples were applied to the gel together with 5 l of Thermo Scientific PageRuler Unstained Broad Range Protein Ladder

This remarkable signaling behavior makes GHK-Cu one of the most studied peptides in regenerative biology research, with particular focus on its role in wound healing and tissue remodeling

Knezevic J, Starchl C, Tmava Berisha A, Amrein K (2020) Thyroid-gut-axis: how does the microbiota influence thyroid function

Park et al., 2019a)

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