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Running multiple peptides simultaneously represents a significant ongoing expense

GABA/glutamate neuron differentiation imbalance and increased AKT/mTOR signaling in CNTNAP2(-/-) cerebral organoids

doi: 10.1038/s41586-022-04432-7 129 LassmannH

Two different reaction mixtures were prepared for each sample to assess hPrx2 and cysteinylated hPrx2 activity: 1 M Prx2 or 1 M Prx2-S-S-Cys (cysteinylated Prx2), 1 M Trx1, 2 nM TrxR1, 300 nM NADPH, and 100 mM H 2 O 2 in TE buffer (50 mM Tris, 1 mM EDTA)