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The cells were incubated with a detection solution of 1 mL per well avoiding light for 20 min at 37 C, and then observed by fluorescence microscopy U-HGLGPS (Olympus, Japan)

Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development

The total levels of glutathione (GSH + GSSG) in plasma were quantified using the Glutathione Colorimetric Detection Kit (Invitrogen, Catalog Number: EIAGSHC)

We should note that methylcobalmin does start to breakdown when exposed to light so it should be kept out of direct light

This process avoids adding external molecules since interferon gamma (IFN-) and tumor necrosis factor-alpha (TNF-), two cytokines associated to the SASP, act as natural adjuvant by promoting an immunoreaction