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The insoluble (pellet) fraction, which contains nuclei, was suspended in ice-cold nuclear extraction reagent and then vortexed at the highest setting for 15 s

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the volatile organic compounds emitted from each sample were concentrated into an adsorption glass tube containing 300 mg Tenax TA (Shimadzu Co., Kyoto, Japan) by purging with pure nitrogen gas at 60 mL/min for 90 min

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