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After removing debris and epithelial cells using 100 and 40 m cell strainers, respectively, ESCs were resuspended in DMEM/F12 containing 10% foetal bovine serum (FBS) (Gibco, New York, USA) and 1% penicillinstreptomycin (Gibco, New York, USA) and cultured in 5% CO 2 at 37 C
doi: 10.3390/ijms20102454 498 BrockmuellerARuiz de PorrasVShakibaeiM
Metabolomic profiling distinction of human nonalcoholic fatty liver disease progression from a common rat model
By estimating the correlations between amplitudes of individual GH secretory events and the average acyl-ghrelin concentration in the 60-minute interval preceding each GH burst, the ghrelin/GH association was more than 3-fold lower in the older group compared with the young adults, thus suggesting that with normal aging, endogenous acyl-ghrelin levels are less tightly linked to GH regulation
