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For severe deficiency, patients get 1,000 mcg daily for the first week

Cells resuspended in the growth medium (GM, DMEM containing 10% fetal bovine serum (FBS, Life Technologies) and 1% anti-anti), and the single cells were seeded in a cell culture flask

B12 is water-soluble, meaning your body excretes what it doesn't need rather than storing it to potentially harmful levels

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The nucleophile provides the electrons to form the new bond and the pi bond of the carbonyl group is broken as it "gets out of the way." The electrons move from this pi bond onto what was the carbonyl oxygen