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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

It is often explored in laboratory settings focused on cellular activity and recovery-related research

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We established a D-galactose-induced aging model in randomly selected male and female mice through intraperitoneal injections of D-galactose (200 mg/kg/day, MCE, HY-N0210), starting at 8 weeks of age for 8 weeks