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Immunoprecipitation The Pierce Classic Magnetic IP/Co-IP Kit (88804, Thermo Fisher Scientific) was used for immunoprecipitation

4, Supplementary Data 1)

With that, they essentially made one of the most powerful glutathione formulations in the list

Specifically, GHK-Cu was found to be least possible to cause skin irritation, while CuCl 2 and Cu(OAc) 2 were found to induce the expression of various skin irritation biomarkers, i.e., IL-1, IL-8, FOSL1, and HSPA1A

J Am Chem Soc 133(50):2016820171 Meng HM, Jin Z, Lv Y, Yang C, Zhang XB, Tan W, Yu RQ (2014) Activatable two-photon fluorescence nanoprobe for bioimaging of glutathione in living cells and tissues