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The cells were grown in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 100 U/ml penicillin and 100 g/ml streptomycin (GenDEPOT, Barker, TX, USA) at 37C in a humidified incubator with 5% CO2

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This step, in turn, constitutes a rate-limiting factor for DNA synthesis ( 2.2 Methionine and mTOR pathway The mTOR serves as a central molecular hub that positively governs anabolic processes while simultaneously exerting negative regulation on certain catabolic pathways (Xie and Proud, 2013)

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