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Prior to analyte injection, recombinant hPD-L1 was covalently immobilized onto a CM5 sensor chip (Cytiva) through standard amine coupling chemistry

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To observe GSH changes on adherent HEK-293 cells dosed with l-Buthionine Sulfoximine (BSO), we used the GSH/GSSG-Glo Assay to monitor total GSH levels (Figure 5)