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Cell culture Human promyelocytic (HL60) cells from ATCC were maintained in RPMI 1640 media containing 10% foetal bovine serum and 200U/ml penicillin and streptomycin at 37 0 C in a humidified atmosphere of 5% CO 2 and 95% air

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Moreover, while microbial genes associated with secondary bile acid biosynthesis showed differential abundance at the early stage, pathways related to LPS biosynthesis and lipid metabolism were abundant at the advanced stage (Wu et al., 2020a)

Milk thistle extract may have an appetite-stimulating effect and support gastric acid production as well as bile flow both of which are relevant for healthy digestion

Compared with FeSAN, pFeSAN possessed a remarkably higher proportion of pyridinic N (Fig