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Why do these exist

To validate our hypothesis regarding the functional importance of R1368, we generated a site-directed mutant by substituting arginine at position 1368 with alanine (R1368A) to disrupt the strong interaction with lactyl-CoA

Structural optimizations, such as replacing the P2 valine with glycine to form Glutamic acid-Glycine-Citrulline (EGCit) (Figure 7a) or designing a non-linear Exo-EEVCit linker (Figure 7b), have proven effective in resisting neutrophil elastase hydrolysis

doi: 10.1038/s41392-023-01471-y 122 ChenJWangXCuiTNiQZhangQZouDet al