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These bacteria are present in soil and water and before the advent of modern farming and improved hygiene, people probably consumed enough food contaminated with these bacteria to cover their B12 needs (Zugravu et al., 2021)

The slides were subjected to final washes with PBS, air-dried and then sealed with anti-fluorescence quenching tablets

The resultant clusters, namely, 31, 4, 19, 50, 35, 20, 36, 42, and 23, were utilized in subsequent stages

Results In the operated rats, at 24 and 72 hr of the reperfusion, the therapy counteracted both early and delayed neural hippocampal damage, achieving full functional recovery (Morris water maze test, inclined beamwalking test, lateral push test)

2.17 TUNEL Cell or tissue sections were fixed with 4% paraformaldehyde at room temperature for 15 min, followed by three washes with phosphate-buffered saline (PBS)